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KMID : 0613820060160040605
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Journal of Life Science
2006 Volume.16 No. 4 p.605 ~ p.611
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Isolation of Bacillus amyloliquefaciens H41 Producing Growth Inhibition Factor against Vibrio anguillarum.
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Kim Young-Hee
Jeong Yong-Kee
Chung Kyung-Tae
Rhu Eun-Ju
Jeong Yu-Jeong
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Abstract
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To investigate the possible use of probiont in fish farming industry, a bacterium with inhibitory effect against Vibrio anguillarum was isolated from gastrointestinal tract of the marine fish of yellow tail. It was identified to be Bacillus amyloliquefaciens H41 based on biochemical and physiological characterization. The optimal growth conditions of the isolated strain were 1% peptone, 1.5% yeast extract, 1% sucrose, 0.5% NaCl, 0.05% MgSO₄ㆍ7H₂0, pH 7.0-8.0, and 20 hr of incubation between 28-35℃ under aeration. The culture supernatant of the isolated strain showed inhibition activity against V. anguillarum. Inhibition activity was cleared by forming a clear zone by a paper-disk method. The maximal production of growth inhibition factor was induced by cultivation under 1% peptone, 1.5% yeast extract, 1% sucrose, 1% NaCl, 0.05% MgSO₄ㆍ7H₂0, pH 7.5 and at 35℃. The highest growth inhibition factor production was observed after 16-24 hr cultivation under aeration. The culture supernatant of the isolated strain showed inhibition activity whereas no inhibition activity was shown from the standard B. amyloliquefaciens KCTC 1724 strain. The growth inhibition affected only against V. anguillarum among other pathogenic Vibrios tested here
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KEYWORD
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Vibrio anguillarum, Bacillus amyloliquefaciens H41, growth inhibition factor
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